OUTPACE cruise (PI: Thierry Moutin and Sophie Bonnet) : February - March 2015		
Contact:		
Cécile Dupouy, Philippe Gérard		
	Centre IRD de Nouméa BP A5 CEDEX Nouvelle Calédonie	
	Tel 687 26 07 29, 687 26 07 21	
	Email : cecile.dupouy@ird.fr, philippe.gerard@ird.fr	
		
Notes:		
1	Samples received in liquid nitrogen at return of the cruise (vol=550 ml) on Whatman GF/F filters. 	
		
2	25mm diameter filters were placed in 7ml of 100% methanol. Extraction time lasted 2 hour. Turner Designs Trilogy Optical kit n°4. Calibration for the OUTPACE series.	
		
3	Analysis by fluorimetry was carried out the same day. Instrument: fluorimeter 	
		
4	Calibration is performed on blank filters	
		
5	The analytical procedure is described in: 	
		Aminot A., Kérouel R., (2004). Hydrologie des écosystèmes marins. Paramètres et analyses. Ed. Ifremer, Méthodes d'anayse en milieu marin, 336 p.
		
6	Detection of Chlorophyll and "pheophytin". Pheophytin is not real pheophytin. Caution: do not compare fluorimetric pigments with HPLC pigments directly.	
	To compare between fluorimetry and HPLC, please use the relationship established by Cecile Dupouy for OUTPACE between Tchla fluorimetry and Tchla (chla + div chla ) by HPLC (r2=0.854, Tchla_HPLC=0.8 x Tchla_fluorimetry) 	
		
		
7	Performance metrics: 	Measurement precision: 10%
		
		
9	Shipboard filtration person: Cécile Dupouy	
	Analysts:  Celine Dimier and Josephine Ras	QA=1 = "good"
		QA=2 = "acceptable"
10	Quality control evaluation of the peaks:	QA=3 = "questionnable"